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Image Search Results
Journal: Frontiers in Microbiology
Article Title: Bacterium-Like Particles Displaying the Rift Valley Fever Virus Gn Head Protein Induces Efficacious Immune Responses in Immunized Mice
doi: 10.3389/fmicb.2022.799942
Figure Lengend Snippet: Identification of the fusion protein. Normal Sf9 cells (A,C) , Sf9 cells infected with 3% recombinant baculovirus for 24 h, cells showed CPE of big and round (B) and were fixed, permeabilized, and stained with rabbit anti-RVFV eGn serum produced in-house and fusion protein is labeled by goat antirabbit IgG/FITC (D) . Identification of the stability of recombinant baculovirus by PCR (E) , lane 1 is Sf9 cells, lane 2 is control baculovirus, and lanes 3 and 4 are the third- and fourth-generation recombinant baculovirus, RVFV Gnhead-PA is 1776 bp. The fusion proteins secreted into the cell supernatant detected by WB (F) , lanes 1 and 2 are a protein marker, lane 3 is Sf9 cells, lane 4 is a mixture of Sf9 cells infected with control baculovirus, the supernatant (lane 5) and cell lysis fragment (lane 6) of the Sf9 cells infected with the recombinant baculovirus. RVFV Gn head-PA is 70 kD.
Article Snippet: The following day, the membrane was washed five times (6 min/wash) with PBST (0.5% Tween-20) and then incubated for 1 h at room temperature with
Techniques: Infection, Recombinant, Staining, Produced, Labeling, Marker, Lysis